As antibiotic resistant bacterial strains are becoming more and more commonly found in hospitals, automated molecular biology solutions will help to tackle the burden of increased testing. The ELITe InGenius full-automation instrument has a wide portfolio of multiplex virus and bacteria testing but it also has several assays for detection of antibiotic-resistant bacteria strains such as Methicillin-resistant Staphylococcus aureus/Staphylococcus aureus (MRSA/SA), carbapenemase-producing Enterobacteriaceae (CRE), and extended-spectrum beta-lactamase (ESBL). Girlich et al. (2018) have evaluated the CRE and ESBL ELITe MGB assays and found an impressive 100% sensitivity and 100% specificity for the CRE ELITe MGB kit and 100% sensitivity and 96,6% specificity for the ESBL ELITe MGB Kit.
The CRE ELITe MGB results were also compared to the results by the French National Reference Center (FNRC), which had identified carbapenem-resistant Enterobacteriaceae genes from the sample pool in France during years 2012-2015. Out of that sample pool, FNRC had confirmed 3320 samples positive for CRE. Based on those findings, researchers expected to have sensitivity of 100% and specificity of 99,58% for CRE ELITe MGB kit in best scenario. Therefore, the final 100% sensitivity and 100% specificity values are excellent.
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